Epidemiology And Pathogenesis

  • Most common primary intraocular malignancy of childhood.
  • Incidence: 1 in 17,000 to 20,000 live births.
  • Median age at diagnosis: 2 years; >90% diagnosed before 5 years.
  • 66-75% present with unilateral tumors; remainder bilateral.
  • Bilateral involvement always heritable; typically presents <1 year of age.
  • Originates from embryonic retina; likely cone photoreceptor precursor cell.
  • Histology exhibits small round blue cells with pathognomonic Flexner-Wintersteiner rosettes.

Genetic Classification

Pathogenesis follows Knudson “two-hit” model of oncogenesis, requiring biallelic inactivation of RB1 tumor suppressor gene on chromosome 13q14.

FeatureHereditary (Heritable)Sporadic (Non-Heritable)
Frequency~50% of cases.~50% of cases.
PresentationBilateral, multifocal, earlier onset (<2 years).Unilateral, unifocal, later onset.
Mutation OriginFirst hit germline; second hit somatic.Both hits somatic within retinal cells.
Second MalignanciesHigh risk (osteosarcoma, soft tissue sarcoma, melanoma).No increased risk.

Note: Approximately 10-15% of unilateral unifocal cases harbor germline RB1 mutations.

Clinical Manifestations

  • Leukocoria (white pupillary reflex): Most common presenting sign.
  • Strabismus: Frequent initial complaint.
  • Advanced signs: Decreased visual acuity, orbital inflammation, hyphema, proptosis, irregular pupil, secondary glaucoma, phthisis bulbi.
  • Trilateral Retinoblastoma: Bilateral disease accompanied by pineal region primitive neuroectodermal tumor (pineoblastoma); occurs in 4-5% of familial cases.
  • 13q Deletion Syndrome: Increased risk; phenotype includes microcephaly, broad forehead, hypertelorism, micrognathia.

Diagnostic Evaluation

  • Examination under anesthesia (EUA): Essential for complete visualization, tumor mapping using RetCam, and optical coherence tomography.
  • Neuroimaging: Orbital and brain Magnetic Resonance Imaging (MRI) assess intraocular extent, optic nerve invasion, and intracranial disease.
  • Avoid biopsy: Strictly contraindicated to prevent extraocular tumor seeding.
  • Avoid Computed Tomography (CT): Minimizes ionizing radiation exposure.

Management Principles

Treatment hierarchy: Cure disease, preserve vision and globe, minimize late effects.

  • Enucleation: Indicated for advanced/massive tumors (Group E) without salvageable vision, or high risk of extraocular spread.
  • Focal Ophthalmic Therapy: Laser photocoagulation or cryotherapy utilized for small tumors.
  • Systemic Chemotherapy: Chemoreduction shrinks tumors prior to focal therapy. Standard regimen includes Carboplatin, Etoposide, Vincristine (CEV).
  • Targeted Chemotherapy: Intra-arterial (melphalan, topotecan, carboplatin via ophthalmic artery) and intravitreal delivery reduce systemic toxicity while maintaining efficacy.
  • Radiation Therapy: External beam radiation strictly avoided in heritable disease due to massive secondary malignancy risk. Episcleral plaque brachytherapy (I-125) serves as a safer, localized alternative.